Klasik Ve Moleküler Genetik Laboratuvar

Ders Kodu:

GBE216

Ders Dönemi:

Bahar

Ders Tipi:

Zorunlu

Kredi:

2

Teori Saati:

0

Uygulama Saati:

1

Laboratuvar Saati:

3

AKTS:

5

Ön Koşul Dersleri:

Biyoloji I

Dersin Dili:

İngilizce

Dersin Koordinatörü:

Gamze Torun Köse

Dersi Verenler:

Gamze Torun Köse

Dersin Amacı:

Co-requisite GBE 212 The major goals in this course are to ensure that the students gain basic genetics and molecular biology background at a practical level, that they have the capability to design and carry out experiments in genetics and bioengineering fields and thus to provide effective solutions to questions and problems that they may face in the future.

Dersin İçeriği:

The course provides the concepts of Mendelian Genetics, Sex Linkage and Crossing Over, Polytene Chromosome, Isolation of DNA and Agarose Gel Electrophoresis, PCR, Real Time PCR, Total RNA Isolation, DNA Sequencing and Chromosome Analysis.

Dersin Öğretim Yöntemleri:

1: Lecture by instructor, 2: Lecture by instructor with class discussion, 3: Problem solving by instructor, 4: Use of simulations, 5: Problem solving assignment, 6: Reading assignment, 7: Laboratory work, 8: Term research paper, 9: Presentation by guest s

Dersin Ölçme Yöntemleri:

A: Written exam, B: Multiple-choice exam C: Take-home quiz, D: Experiment report, E: Homework, F: Project, G: Presentation by student, H: …

Dikey Sekmeler

Ders Tanimlari

Dersin Öğrenme Çıktıları

Course Learning Outcomes	Detailed Program Outcomes	Teaching Methods	Assessment Methods
Ability to interpret Mendelian Genetics	1, 3	1, 3	A, B, C,D
Ability to implement Monohybrid and Dihybrid Cross	1, 3, 7	1, 3, 7	A, B, C,D
Ability to implement Sex Linkage and Crossing over	1, 3, 7	1, 3, 7	A, B, C,D
Ability to interpret Polytene Chromosome	1, 7	1, 7	A, B, C,D
Ability to implement DNA Isolation by Agarose Gel Electrophoresis	1, 7	1, 7	A, B, C,D
Ability to use PCR	1, 7	1, 7	A, B, C,D
Ability to use Real Time PCR	1, 7	1, 7	A, B, C,D
Ability to implement Total RNA Isolation	1, 7	1, 7	A, B, C,D
Ability to use DNA Sequencing	1, 7	1, 7	A, B, C,D
Ability to implement Chromosome Analysis	1, 7	1, 7	A, B, C,D

Dersin Akışı

COURSE CONTENT
Week	Topics	Study Materials
1	Introduction to Mendelian Genetics (16 Sept, Ezgi İrem Bektaş)	3
2	Monohybrid and Dihybrid Cross (23 Sept, Nisa İrem Büyük)	3
3	Sex Linkage and Crossing Over (30 Sept, Kardelen Tüfekçi)	5
4	Polytene Chromosome (7 Oct, Gülçin Delal Nozhatzadeh)	12
5	Isolation of DNA and Agarose Gel Electrophoresis (14 Oct, Derya Burukçu)	10
6	MIDTERM I (21 Oct, Labs 1-5)
7	PCR (28 Oct, Sümbül Yıldırım-Merve Uslu)	20
8	Real Time PCR (4 Nov, Derya Burukçu)	20
9	Total RNA isolation (11 Nov, Gülçin Delal Nozhatzadeh)	20
10	DNA Sequencing (18 Nov, Batoul Yousef)	20
11	Chromosome Analysis (25 Nov, Zehra İnkaya- Esra Albayrak)	20
12	FINAL (2 Dec, Labs 1-11)	16

Kaynaklar

RECOMMENDED SOURCES
Textbook	Concepts of Genetics- W.S.Klug, M.R.Cummings, C.A.Spencer, M.A.Palladino
Additional Resources	Molecular biology of the gene-Watson, Baker, Bell, Gann, Levine,

Materyal Paylaşımı

MATERIAL SHARING
Documents	Handouts
Assignments
Exams	9 Quizes, 10 Reports, 1 Midterm, 1 Final

Değerlendirme Sistemi

ASSESSMENT
IN-TERM STUDIES	NUMBER	PERCENTAGE
Quizes	9	15 %
Reports	10	30 %
Midterm	1	25 %
Total		70 %
CONTRIBUTION OF FINAL EXAMINATION TO OVERALL GRADE		30 %
LAB PERFORMANCE CONTRIBUTION OF IN-TERM STUDIES TO OVERALL GRADE		±10 %
Total		100

Dersin Program Çıktılarına Katkısı

COURSE'S CONTRIBUTION TO PROGRAM OUTCOMES
No	Program Learning Outcomes	check √
1a	Adequate knowledge in mathematics, science and engineering subjects pertaining to the relevant discipline,	√
1b	Ability to use theoretical and applied knowledge in these areas in complex engineering problems.
2a	Ability to identify, formulate, and solve complex engineering problems,
2b	Ability to select and apply proper analysis and modeling methods for this purpose.	√
3a	Ability to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the desired result,	√
3b	Ability to apply modern design methods for this purpose.	√
4a	Ability to devise, select and use modern techniques and tools needed for analyzing and solving complex problems encountered in engineering practice.	√
4b	Ability to employ information technologies effectively.
5a	Ability to design experiments for investigating complex engineering problems or discipline specific research questions,	√
5b	Ability to conduct experiments, gather data, analyze and interpret results for investigating complex engineering problems or discipline specific research questions.
6a	Ability to work efficiently in intra-disciplinary teams,	√
6b	Ability to work efficiently in multi-disciplinary teams,
6c	Ability to work individually.	√
7a	Ability to communicate effectively in Turkish, both orally and in writing,
7b	Knowledge of a minimum of one foreign language,
7c	Ability to write effective reports and comprehend written reports, prepare design and production reports,	√
7d	Ability to make effective presentations,
7e	Ability to give and receive clear and intelligible instructions.	√
8a	Recognition of the need for lifelong learning, ability to access information, ability to follow developments in science and technology,	√
8b	Ability to continue to educate him/herself.	√
9a	Consciousness to behave according to ethical principles and professional and ethical responsibility.	√
9b	Knowledge on standards used in engineering practice.	√
10a	Knowledge about business life practices such as project management, risk management, change management.
10b	Awareness in entrepreneurship and innovation.
10c	Knowledge about sustainable development.
11a	Knowledge about the global and social effects of engineering practices on health, environment, and safety,	√
11b	Knowledge about contemporary issues of the century reflected into the field of engineering.
11c	Awareness of the legal consequences of engineering solutions.

AKTS İş Yükü Tablosu

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Course Duration	10	4	40
Hours for off-the-classroom study (Pre-study, practice)	10	4	40
Mid-term examination	2	2	4
Experiment	10	4	40
Final examination	1	3	3
Total Work Load			127
Total Work Load / 25 (h)			5,08
ECTS Credit of the Course			5

Arama formu

Klasik Ve Moleküler Genetik Laboratuvar

Dikey Sekmeler

Ders Tanimlari