Biochemistry II

Course Code:

GBE302

Course Period:

Spring

Course Type:

Core

Credits:

4

Theoric:

2

Practice:

1

Laboratory Hour:

3

ECTS:

9

Prerequisite Courses:

Biochemistry I

Course Language:

English

Course Coordinator:

Dilek Telci Temeltas

Courses given by:

Dilek Telci Temeltas

Course Objectives:

• To understand how carbohydrate metabolism, lipid metabolism and amino acid metabolism work together to sustain body's equilibrium or homeostasis. • To foster the students the ability to apply the mathematics, science and engineering (in particular, bioengineering) knowhow that they are going to require in either academic or industrial lives. • To ensure that the students gain biochemistry background at a theoretical as well as practical level, that they have the capability to design and carry out experiments in genetics and bioengineering fields and thus to provide effective solutions to questions and problems that they may face in the future. • To train individuals with professional ethics and social conscience, who complies with professional ethics in contemporary topics in applications, with a mission to avoid any verbal and hands-on exercise that would jeopardize the environment and human health.

Course Content:

Laws of thermodynamics; ATP and energy; cellular metabolism and fermentation; glycolysis; TCA cycle; oxidative phosphorylation; photosynthesis; fatty acid metabolism; metabolism of nitrogenous compounds: hormonal regulation and integration of mammalian metabolism. Selected biochemistry experiments in the lab section parallel to the course content.

Course Methodology:

1: Lecture by instructor, 2: Lecture by instructor with class discussion, 3: Problem solving by instructor, 4: Use of simulations, 5: Problem solving assignment, 6: Reading assignment, 7: Laboratory work, 8: Term research paper, 9: Presentation by guest s

Course Evaluation Methods:

A: Written exam, B: Multiple-choice exam C: Take-home quiz, D: Experiment report, E: Homework, F: Project, G: Presentation by student, H: …

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Course Learning Outcomes

Course Learning Outcomes	Detailed Program Outcomes	Teaching Methods	Assessment Methods
The ability to understand metabolic pathways of glucose, fatty acids and nitrogenous compounds	1a, 1b, 8a, 8b, 9a, 7a, 7b	1,2,7	A, D
The ability to understand and describe at fundamentals of least two biosynthesis and catabolism pathways.	1a, 1b, 8a, 8b, 7a, 7b	1,2,7	A, D
The ability to understand the working principle of electron transport chain.	1a, 1b, 8a, 8b, 7a, 7b	1,2,7	A, D
The ability to design and perform independent experiments in biochemistry as well as to analyze and interpret the experimental results.	2a, 2b, 3a, 4a, 5a, 5b, 6a, 6b, 6c, , 7a, 7b, 9	1,2,7	A, D
The ability to isolate mitochondria and chloroplast from organic sources.	6a, 6c, , 7a, 7b	1,2,7	A, D
The ability to analyze the hexose content in solutions.	6a, 6c, , 7a, 7b,	1,2,7	A, D

Course Flow

COURSE CONTENT

Week

Topics

Study Materials

1

09.09.2019 Introduction to Metabolism

Text Book and Lecture Slides

2

16.09.2019 Introduction to Metabolism

Date	Laboratory Sessions
19.09.2019	Alcohol Fermentation

Text Book, Lecture Slides, Laboratory Manual

3

23.10.2019 Carbohydrate Metabolism I: Anaerobic Processes in Generating Metabolic Energy

Date	Laboratory Sessions
26.09.2019	Identification of Disaccharide Hydrolases in Yeast

Text Book, Lecture Slides, Laboratory Manual

4

30.09.2019 Oxidative Process: Citric Acid Cycle and Pentose Phosphate Pathway

Date	Laboratory Sessions
19.09.2019	Starch Hydrolysis by Amylase

Text Book, Lecture Slides, Laboratory Manual

5

07.10.2019 Oxidative Process: Citric Acid Cycle and Pentose Phosphate Pathway

Date	Laboratory Sessions
10.10.2019	Review Class for Lecture

Text Book and Lecture Slides

6

14.10.2019 Electron Transport, Oxidative Phosphorylation, and Oxygen Metabolism

Date	Laboratory Sessions
17.10.2019	Cellulose Degradation

Text Book, Lecture Slides, Laboratory Manual

7

Date	Laboratory Sessions
24.10.2019	Midterm I

25.10. 2019 Carbohydrate Metabolism II: Biosynthesis

Text Book and Lecture Slides

8

28.10.2019 Carbohydrate Metabolism II: Biosynthesis and Photosynthesis

Date	Laboratory Sessions
31.10.2019	Isolation of Mitochondria

Text Book, Lecture Slides, Laboratory Manual

9

04.11.2019 Lipid Metabolism I: Fatty Acids, Triacylglycerols, and Lipoproteins

Date	Laboratory Sessions
07.11.2019	Isolation of Chloroplasts from Spinach Leaves and analysis of chlorophyll content; The Hill Reaction

Text Book, Lecture Slides, Laboratory Manual

10

11.11.2019 Lipid Metabolism I: Fatty Acids, Triacylglycerols, and Lipoproteins

Date	Laboratory Sessions
07.11.2019	Review Class for Lecture

Text Book and Lecture Slides

11

Date	Laboratory Sessions
21.11.2019	Midterm II

22.11.2019 Lipid Metabolism II: Membrane Lipids, Steroids,

Text Book and Lecture Slides

12

25.11.2019 Lipid Metabolism II: Membrane Lipids, Steroids

Date	Laboratory Sessions
28.11.2019	Characterization of a Serine Protease

Text Book, Lecture Slides, Laboratory Manual

13

2.12.2019 Metabolism of Nitrogenous Compounds: Principles of Biosynthesis, Utilization, and Turnover

Date	Laboratory Sessions
05.12.2019	SDS-page analysis of proteins

Text Book, Lecture Slides, Laboratory Manual

14

09.12.2019 Hormonal Regulation and Integration of mammalian metabolism

Date	Laboratory Sessions
12.12.2019	Laboratory Practical Exam

Text Book and Lecture Slides

Recommended Sources

RECOMMENDED SOURCES
Textbook	Biochemistry, Matthews, Van Holde, Ahern, 4th Edition (International Edition).
Additional Resources	In addition, other biochemistry text books can be used as possible supplementary reading material.

Material Sharing

MATERIAL SHARING
Documents	Lecture Notes can be downloaded from Moodle.
Assignments	Laboratory Reports will be submitted to Turnitin for evaluation one week after the laboratory practicals.

Assessment

ASSESSMENT
IN-TERM STUDIES	NUMBER	PERCENTAGE
Midterm	2	15 x2 = 30
Laboratory	8	35
Quizes & Assigments (bonus)		5
Total
CONTRIBUTION OF FINAL EXAMINATION TO OVERALL GRADE		35
CONTRIBUTION OF IN-TERM STUDIES TO OVERALL GRADE		65
Total		100

Course’s Contribution to Program

COURSE'S CONTRIBUTION TO PROGRAM OUTCOMES
No	Program Learning Outcomes	check √
1a	Adequate knowledge in mathematics, science and engineering subjects pertaining to the relevant discipline,	√
1b	Ability to use theoretical and applied knowledge in these areas in complex engineering problems.	√
2a	Ability to identify, formulate, and solve complex engineering problems,	√
2b	Ability to select and apply proper analysis and modeling methods for this purpose.	√
3a	Ability to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the desired result,	√
3b	Ability to apply modern design methods for this purpose.
4a	Ability to devise, select and use modern techniques and tools needed for analyzing and solving complex problems encountered in engineering practice.	√
4b	Ability to employ information technologies effectively.
5a	Ability to design experiments for investigating complex engineering problems or discipline specific research questions,	√
5b	Ability to conduct experiments, gather data, analyze and interpret results for investigating complex engineering problems or discipline specific research questions.	√
6a	Ability to work efficiently in intra-disciplinary teams,	√
6b	Ability to work efficiently in multi-disciplinary teams,	√
6c	Ability to work individually.	√
7a	Ability to communicate effectively in Turkish, both orally and in writing,	√
7b	Knowledge of a minimum of one foreign language,	√
7c	Ability to write effective reports and comprehend written reports, prepare design and production reports,	√
7d	Ability to make effective presentations,
7e	Ability to give and receive clear and intelligible instructions.
8a	Recognition of the need for lifelong learning, ability to access information, ability to follow developments in science and technology,	√
8b	Ability to continue to educate him/herself.	√
9a	Consciousness to behave according to ethical principles and professional and ethical responsibility.	√
9b	Knowledge on standards used in engineering practice.
10a	Knowledge about business life practices such as project management, risk management, change management.
10b	Awareness in entrepreneurship and innovation.
10c	Knowledge about sustainable development.
11a	Knowledge about the global and social effects of engineering practices on health, environment, and safety,
11b	Knowledge about contemporary issues of the century reflected into the field of engineering.
11c	Awareness of the legal consequences of engineering solutions.

ECTS

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
Activities	Quantity	Duration (Hour)	Total Workload (Hour)
Course Duration	14	3	42
Hours for off-the-classroom study (Pre-study, practice)	14	7	98
Mid-term examination	2	3	6
Experiment	8	3	24
Final Examination	1	3	3
Total Work Load			215
Total Work Load / 25 (h)			8.6
ECTS Credit of the Course			9

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Biochemistry II

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